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Glick, Benjamin

One or more keywords matched the following properties of Glick, Benjamin

Property	Value
keywords	Organelles
overview	Our goal is to understand the processes that generate compartments of the secretory pathway, including ER exit sites (ERES; also known as transitional ER or tER sites) and the cisternae of the Golgi apparatus. Self-organization models provide the conceptual framework. Specifically, we postulate that ERES are generated together with early Golgi cisternae by an integrated self-organization pathway, and that early cisternae progressively mature into late cisternae. For exploring these ideas, our main experimental system is a pair of budding yeasts. In Saccharomyces cerevisiae, Golgi cisternae are dispersed throughout the cytoplasm and the ER contains multiple small ERES, whereas in Pichia pastoris, ordered Golgi stacks are located next to large, stable ERES. These two yeasts have complementary advantages for testing specific hypotheses about the secretory pathway. We use a combination of genetics, molecular biology, 4D confocal microscopy, and electron tomography. This work is revealing evolutionarily conserved principles of cellular organization. A second project in the lab involves optimizing fluorescent proteins, including the red fluorescent protein DsRed. Wild-type DsRed matures very slowly. We overcame this problem by using directed evolution to create the first rapidly maturing DsRed variants, one of which is marketed commercially as DsRed-Express. More recent work yielded a noncytotoxic variant called DsRed-Express2, as well as a far-red variant called E2-Crimson. These engineering efforts inspired a basic research project in which we clarified the pathway of DsRed chromophore formation. Current efforts are focused on creating improved monomeric green and red fluorescent proteins.

Property

Value

keywords

Organelles

overview

Our goal is to understand the processes that generate compartments of the secretory pathway, including ER exit sites (ERES; also known as transitional ER or tER sites) and the cisternae of the Golgi apparatus. Self-organization models provide the conceptual framework. Specifically, we postulate that ERES are generated together with early Golgi cisternae by an integrated self-organization pathway, and that early cisternae progressively mature into late cisternae. For exploring these ideas, our main experimental system is a pair of budding yeasts. In Saccharomyces cerevisiae, Golgi cisternae are dispersed throughout the cytoplasm and the ER contains multiple small ERES, whereas in Pichia pastoris, ordered Golgi stacks are located next to large, stable ERES. These two yeasts have complementary advantages for testing specific hypotheses about the secretory pathway. We use a combination of genetics, molecular biology, 4D confocal microscopy, and electron tomography. This work is revealing evolutionarily conserved principles of cellular organization. A second project in the lab involves optimizing fluorescent proteins, including the red fluorescent protein DsRed. Wild-type DsRed matures very slowly. We overcame this problem by using directed evolution to create the first rapidly maturing DsRed variants, one of which is marketed commercially as DsRed-Express. More recent work yielded a noncytotoxic variant called DsRed-Express2, as well as a far-red variant called E2-Crimson. These engineering efforts inspired a basic research project in which we clarified the pathway of DsRed chromophore formation. Current efforts are focused on creating improved monomeric green and red fluorescent proteins.

One or more keywords matched the following items that are connected to Glick, Benjamin

Item Type	Name
Concept	Actins
Concept	Alcohol Oxidoreductases
Concept	Acetyl Coenzyme A
Concept	Acetylation
Concept	Adrenal Gland Neoplasms
Concept	Ethanol
Concept	Autophagy
Concept	Mitochondrial ADP, ATP Translocases
Concept	Acyl Coenzyme A
Concept	Aluminum
Concept	Algorithms
Concept	Alcohols
Concept	Alleles
Concept	Amino Acid Sequence
Concept	Aldehyde Oxidoreductases
Concept	Amino Acid Isomerases
Concept	Acetyltransferases
Concept	Carboxypeptidases
Concept	Animals
Concept	Animals, Newborn
Concept	Chromosomes, Human, Pair 6
Concept	Arthrogryposis
Concept	Antibodies, Monoclonal
Concept	Cnidaria
Concept	Base Sequence
Concept	Autoantigens
Concept	Dithionitrobenzoic Acid
Concept	Binding Sites
Concept	Biophysics
Concept	Carps
Concept	Cattle
Concept	Cell Division
Concept	Coenzyme A
Concept	Cell Transformation, Viral
Concept	Centrifugation, Density Gradient
Concept	Centromere
Concept	Chromosome Mapping
Concept	Codon
Concept	Fungi
Concept	Genetic Vectors
Concept	Golgi Apparatus
Concept	Cricetulus
Concept	Clathrin
Concept	Zea mays
Concept	DNA, Mitochondrial
Concept	Detergents
Concept	Insulin
Concept	Saccharomycetales
Concept	Database Management Systems
Concept	Dictyostelium
Concept	DNA, Fungal
Concept	DNA, Neoplasm
Concept	Electrophoresis, Polyacrylamide Gel
Concept	Lac Operon
Concept	Manganese
Concept	Mannitol
Concept	Ethylmaleimide
Concept	Congresses as Topic
Concept	Diagnostic Imaging
Concept	Electron Transport Complex IV
Concept	Disease Susceptibility
Concept	Drosophila melanogaster
Concept	Fibroblasts
Concept	Introns
Concept	Models, Chemical
Concept	Models, Structural
Concept	Fluorescence
Concept	Gene Expression Regulation
Concept	Genetic Techniques
Concept	Genes, Lethal
Concept	Guanosine Triphosphate
Concept	Fluorides
Concept	Freezing
Concept	Genes, Recessive
Concept	Oncogenes
Concept	Genetic Engineering
Concept	Hydrogen Peroxide
Concept	Liver Neoplasms
Concept	Enzyme Induction
Concept	Escherichia coli
Concept	Feedback
Concept	Flow Cytometry
Concept	Fluorescent Antibody Technique
Concept	Gene Amplification
Concept	Heme
Concept	Luminescent Measurements
Concept	Membrane Glycoproteins
Concept	Carcinoma, Hepatocellular
Concept	Metalloendopeptidases
Concept	Hydrolysis
Concept	Models, Molecular
Concept	Phosphatidate Phosphatase
Concept	Phospholipids
Concept	Mice, Transgenic
Concept	Microbodies
Concept	Neurospora crassa
Concept	Protein Denaturation
Concept	Proton-Translocating ATPases
Concept	Microcomputers
Concept	Mutation
Concept	Cricetinae
Concept	Heat-Shock Proteins
Concept	L-Lactate Dehydrogenase
Concept	Ovary
Concept	Oxidation-Reduction
Concept	Pedigree
Concept	Membrane Fusion
Concept	Microscopy, Electron
Concept	Neurospora
Concept	Oxygen
Concept	Pichia
Concept	Plasmids
Concept	Protein Conformation
Concept	Protein Processing, Post-Translational
Concept	Proto-Oncogene Proteins
Concept	Glutamine
Concept	Radioisotope Dilution Technique
Concept	Rana pipiens
Concept	Ribulose-Bisphosphate Carboxylase
Concept	Schizosaccharomyces
Concept	Humans
Concept	Stress, Mechanical
Concept	Sulfur Radioisotopes
Concept	Software
Concept	Intracellular Membranes
Concept	Sequence Tagged Sites
Concept	Xenopus laevis
Concept	Yeasts
Concept	Colony Count, Microbial
Concept	Protein Engineering
Concept	Oligonucleotide Probes
Concept	Lysosomes
Concept	Gene Library
Concept	Gene Expression Regulation, Fungal
Concept	Guanine Nucleotide Exchange Factors
Concept	Coatomer Protein
Concept	Protein Structure, Quaternary
Concept	Mitogen-Activated Protein Kinases
Concept	Guanosine 5'-O-(3-Thiotriphosphate)
Concept	Myosin Type II
Concept	Vacuolar Proton-Translocating ATPases
Concept	Cryopreservation
Concept	Mutagenesis
Concept	Mitochondria
Concept	Genomic Library
Concept	Chromosomes, Fungal
Concept	Nucleotide Transport Proteins
Concept	Mutagenesis, Site-Directed
Concept	Sequence Alignment
Concept	Microscopy, Immunoelectron
Concept	RNA Interference
Concept	Alternative Splicing
Concept	Sequence Analysis, DNA
Concept	Protein Structure, Tertiary
Concept	Genes, Reporter
Concept	Morphogenesis
Concept	Myosins
Concept	NADH Dehydrogenase
Concept	L-Lactate Dehydrogenase (Cytochrome)
Concept	Gene Transfer Techniques
Concept	Gene Deletion
Concept	Sequence Deletion
Concept	Crystallography, X-Ray
Concept	Cell Proliferation
Concept	HSP70 Heat-Shock Proteins
Concept	Microscopy, Video
Concept	Qa-SNARE Proteins
Concept	Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins
Concept	Calcium Signaling
Concept	Mitochondrial Proton-Translocating ATPases
Concept	Drosophila Proteins
Concept	Chaperonins
Concept	Myosin Heavy Chains
Concept	GTP Phosphohydrolases
Concept	rab GTP-Binding Proteins
Concept	Octoxynol
Concept	Green Fluorescent Proteins
Concept	History, 21st Century
Concept	Proteomics
Concept	N-Ethylmaleimide-Sensitive Proteins
Concept	Endopeptidase K
Concept	Microscopy, Confocal
Concept	SNARE Proteins
Concept	Chaperonin 60
Concept	Internet
Concept	Peroxisomes
Concept	Entropy
Concept	trans-Golgi Network
Concept	Peptidylprolyl Isomerase
Concept	Schizosaccharomyces pombe Proteins
Concept	Polyethylene Glycols
Concept	Nuclear Pore Complex Proteins
Concept	Saccharomyces cerevisiae Proteins
Concept	Promoter Regions, Genetic
Concept	Mice
Concept	Aryl Hydrocarbon Receptor Nuclear Translocator
Concept	Receptors, Nicotinic
Concept	Endosomes
Concept	Rats, Inbred Strains
Concept	Ribonucleases
Concept	Saccharomyces cerevisiae
Concept	Spectrometry, Fluorescence
Concept	Multiprotein Complexes
Concept	Rats
Concept	Insulin-Secreting Cells
Concept	Temperature
Concept	Immunoblotting
Concept	Tetrahymena
Concept	Thiazoles
Concept	Thiosulfate Sulfurtransferase
Concept	Open Reading Frames
Concept	Genome, Fungal
Concept	Transfection
Concept	Vertebrates
Concept	Blotting, Southern
Concept	GTP-Binding Proteins
Concept	Aluminum Compounds
Concept	Coat Protein Complex I
Concept	Imaging, Three-Dimensional
Concept	Gene Targeting
Concept	Myosin Type V
Concept	Bioreactors
Concept	Monomeric GTP-Binding Proteins
Concept	GTPase-Activating Proteins
Concept	Two-Hybrid System Techniques
Concept	ADP-Ribosylation Factor 1
Concept	Protein Sorting Signals
Concept	History, 20th Century
Concept	Transcriptome
Concept	Genome-Wide Association Study
Concept	Haploinsufficiency
Concept	Molecular Sequence Annotation
Concept	Microscopy, Fluorescence, Multiphoton
Concept	Synthetic Biology
Concept	Methanol
Concept	Biochemistry
Concept	Cell Survival
Concept	Cloning, Molecular
Concept	Color
Concept	Digitonin
Concept	DNA Restriction Enzymes
Concept	Genes, Fungal
Concept	Glucose
Concept	Histidine
Concept	Kinetics
Concept	Membrane Potentials
Concept	Microscopy, Fluorescence
Concept	Models, Biological
Concept	Nuclear Envelope
Concept	Osmotic Pressure
Concept	Palmitoyl Coenzyme A
Concept	Recombinant Fusion Proteins
Concept	Serine Endopeptidases
Concept	Staining and Labeling
Concept	Information Storage and Retrieval
Concept	Mutagenesis, Insertional
Concept	Porins
Concept	Chaperonin 10
Concept	Computational Biology
Concept	Membrane Transport Proteins
Concept	Databases, Nucleic Acid
Concept	Mitochondrial Membrane Transport Proteins
Concept	Vesicular Transport Proteins
Concept	Adaptor Proteins, Vesicular Transport
Concept	Nuclear Lamina
Concept	Cathepsin A
Academic Article	Translocation arrest of an intramitochondrial sorting signal next to Tim11 at the inner-membrane import site.
Academic Article	Pathways and energetics of mitochondrial protein import in Saccharomyces cerevisiae.
Academic Article	A yeast t-SNARE involved in endocytosis.
Academic Article	Active unfolding of precursor proteins during mitochondrial protein import.
Academic Article	The curious status of the Golgi apparatus.
Academic Article	Golgi structure correlates with transitional endoplasmic reticulum organization in Pichia pastoris and Saccharomyces cerevisiae.
Academic Article	Organization of the Golgi apparatus.
Academic Article	Barreling through the outer membrane.
Academic Article	ER export: more than one way out.
Academic Article	A versatile set of vectors for constitutive and regulated gene expression in Pichia pastoris.
Academic Article	Deconstructing Golgi inheritance.
Academic Article	Monitoring changes in the subcellular location of proteins in S. cerevisiae.
Academic Article	The transitional ER localization mechanism of Pichia pastoris Sec12.
Academic Article	Vector for pop-in/pop-out gene replacement in Pichia pastoris.
Academic Article	De novo formation of transitional ER sites and Golgi structures in Pichia pastoris.
Academic Article	The mechanisms of vesicle budding and fusion.
Academic Article	Sec16 is a determinant of transitional ER organization.
Academic Article	MICA: desktop software for comprehensive searching of DNA databases.
Academic Article	Structural rearrangements near the chromophore influence the maturation speed and brightness of DsRed variants.
Academic Article	Mitochondrial protein import.
Academic Article	Let there be order.
Academic Article	Protein import into isolated yeast mitochondria.
Academic Article	Cdc1p is an endoplasmic reticulum-localized putative lipid phosphatase that affects Golgi inheritance and actin polarization by activating Ca2+ signaling.
Academic Article	Golgi inheritance in small buds of Saccharomyces cerevisiae is linked to endoplasmic reticulum inheritance.
Academic Article	Fatty acylation promotes fusion of transport vesicles with Golgi cisternae.
Academic Article	A role for GTP-binding proteins in vesicular transport through the Golgi complex.
Academic Article	Noncytotoxic orange and red/green derivatives of DsRed-Express2 for whole-cell labeling.
Academic Article	A rapidly maturing far-red derivative of DsRed-Express2 for whole-cell labeling.
Academic Article	Two mammalian Sec16 homologues have nonredundant functions in endoplasmic reticulum (ER) export and transitional ER organization.
Academic Article	Vesicular transport between the endoplasmic reticulum and the Golgi stack requires the NEM-sensitive fusion protein.
Academic Article	Journeys through the Golgi--taking stock in a new era.
Academic Article	Chromophore formation in DsRed occurs by a branched pathway.
Academic Article	Sec12 binds to Sec16 at transitional ER sites.
Academic Article	Components responsible for transport between successive Golgi cisternae are highly conserved in evolution.
Academic Article	Purification of an N-ethylmaleimide-sensitive protein catalyzing vesicular transport.
Academic Article	Sequential intermediates in the pathway of intercompartmental transport in a cell-free system.
Academic Article	Fatty acyl-coenzyme A is required for budding of transport vesicles from Golgi cisternae.
Academic Article	Dynamic interaction between Isp45 and mitochondrial hsp70 in the protein import system of the yeast mitochondrial inner membrane.
Academic Article	The yeast Golgi apparatus: insights and mysteries.
Academic Article	The yeast GRASP Grh1 colocalizes with COPII and is dispensable for organizing the secretory pathway.
Academic Article	A new type of coated vesicular carrier that appears not to contain clathrin: its possible role in protein transport within the Golgi stack.
Academic Article	Role of an N-ethylmaleimide-sensitive transport component in promoting fusion of transport vesicles with cisternae of the Golgi stack.
Academic Article	Noncytotoxic DsRed derivatives for whole-cell labeling.
Academic Article	Yeast and mammals utilize similar cytosolic components to drive protein transport through the Golgi complex.
Academic Article	The budding yeast Pichia pastoris has a novel Sec23p homolog.
Academic Article	Cytochromes c1 and b2 are sorted to the intermembrane space of yeast mitochondria by a stop-transfer mechanism.
Academic Article	Fluorescence microscopy and thin-section electron microscopy.
Academic Article	A C-terminally-anchored Golgi protein is inserted into the endoplasmic reticulum and then transported to the Golgi apparatus.
Academic Article	Protein import into mitochondria: the requirement for external ATP is precursor-specific whereas intramitochondrial ATP is universally needed for translocation into the matrix.
Academic Article	Mutation of SYNE-1, encoding an essential component of the nuclear lamina, is responsible for autosomal recessive arthrogryposis.
Academic Article	Membrane traffic within the Golgi apparatus.
Academic Article	Organelle structure and biogenesis.
Academic Article	Involvement of GTP-binding "G" proteins in transport through the Golgi stack.
Academic Article	Cloning and disruption of the gene encoding yeast mitochondrial chaperonin 10, the homolog of E. coli groES.
Academic Article	Dynamics of transitional endoplasmic reticulum sites in vertebrate cells.
Academic Article	Tagging Hansenula polymorpha genes by random integration of linear DNA fragments (RALF).
Academic Article	Brighter reporter genes from multimerized fluorescent proteins.
Academic Article	Identification of pexophagy genes by restriction enzyme-mediated integration.
Academic Article	Possible role for fatty acyl-coenzyme A in intracellular protein transport.
Academic Article	Can Hsp70 proteins act as force-generating motors?
Academic Article	Sec16 influences transitional ER sites by regulating rather than organizing COPII.
Academic Article	Integrated self-organization of transitional ER and early Golgi compartments.
Academic Article	Golgi compartmentation and identity.
Academic Article	GenoLIB: a database of biological parts derived from a library of common plasmid features.
Academic Article	Gottfried Schatz (1936-2015)-mitochondrial pioneer and ambassador for science.
Academic Article	Structure and expression of amplified cKi-ras gene sequences in Y1 mouse adrenal tumor cells.
Academic Article	The energetics of protein import into mitochondria.
Academic Article	Protein import into mitochondria: two systems acting in tandem?
Academic Article	4D Confocal Imaging of Yeast Organelles.
Academic Article	Refined Pichia pastoris reference genome sequence.
Academic Article	Budding Yeast Has a Minimal Endomembrane System.
Academic Article	Visualizing Secretory Cargo Transport in Budding Yeast.
Academic Article	An improved secretion signal enhances the secretion of model proteins from Pichia pastoris.
Academic Article	Maturation-driven transport and AP-1-dependent recycling of a secretory cargo in the Golgi.
Academic Article	4D Microscopy of Yeast.
Academic Article	A Kinetic View of Membrane Traffic Pathways Can Transcend the Classical View of Golgi Compartments.
Academic Article	ER arrival sites associate with ER exit sites to create bidirectional transport portals.
Academic Article	A photostable monomeric superfolder green fluorescent protein.
Academic Article	A microscopy-based kinetic analysis of yeast vacuolar protein sorting.
Academic Article	Bioreactor-scale cell performance and protein production can be substantially increased by using a secretion signal that drives co-translational translocation in Pichia pastoris.
Academic Article	ESCargo: a regulatable fluorescent secretory cargo for diverse model organisms.
Academic Article	Acetyl-CoA flux from the cytosol to the ER regulates engagement and quality of the secretory pathway.
Academic Article	TRAPP structures reveal the big picture.
Academic Article	A General Method to Improve Fluorophores Using Deuterated Auxochromes.
Academic Article	Activity-dependent Golgi satellite formation in dendrites reshapes the neuronal surface glycoproteome.
Academic Article	Clathrin adaptors mediate two sequential pathways of intra-Golgi recycling.
Academic Article	Editorial overview: Membranes in perpetual motion.
Concept	SEC Translocation Channels
Grant	The Transitional ER-Golgi System in Budding Yeasts
Grant	Optimizing and Analyzing Fluorescent Proteins
Grant	Molecular and Cellular Biology Training
Grant	Electron Microscope for a Multi-User Core Facility
Grant	Role of the AP-1 Adaptor in Intra-Golgi Recycling
Grant	Dissecting the functions of yeast COPI
Grant	Optimizing DsRed
Grant	2007 Molecular Membrane Biology GRC
Academic Article	Yellow and oxidation-resistant derivatives of a monomeric superfolder GFP.

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